Table 2. Details of the expression of recombinant nepenthesin 1.

Source organism	N. gracilis
Forward primer	CATATGACGTCAAGAACAGCTC
Reverse primer	AAGCTTTCACGACGCACCACATTG
Cloning vector	pBSSK− (Invitrogen, USA)
Expression vector	pET-21a (Invitrogen, USA)
Expression host	E. coli C41 (DE3) (Lucigen, USA)
Complete amino-acid sequence of the construct produced†	TSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCGAS

^†The propeptide sequence is underlined.