Table 4. Crystallization conditions of rNep1–pepstatin optimized from conditions 2 and 5 of the low-pH crystallization screen.

	Condition (i)	Condition (ii)
Method	Hanging-drop vapour diffusion	Hanging-drop vapour diffusion
Plate type	EasyXtal 15-well tool (Qiagen)	EasyXtal 15-well tool (Qiagen)
Temperature (K)	291	291
Protein concentration (mg ml−1)	10	10
Buffer composition of protein solution	0.05 M glycine pH 2.5	0.05 M glycine pH 2.5
Composition of reservoir solution	25%(w/v) PEG 3350, 0.1 M glycine pH 2.5	2 M ammonium sulfate, 0.1 M glycine pH 2.5, 0.025%(v/v) dichloromethane
Volume and ratio of drop (protein:reservoir solution)	1.5 µl (2:1)	1 µl (1:1)
Volume of reservoir (µl)	500	500