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. 2016 Jan 1;72(Pt 1):29–35. doi: 10.1107/S2053230X15023390

Table 2. Crystallization.

Method Vapour diffusion, sitting drop
Plate type MRC crystallization plate, SWISSCI MRC 2 Well (Jena Bioscience)
Temperature (K) 288
Protein concentration (mg ml−1) 19
Buffer composition of protein solution 20 mM Tris pH 8.0
Composition of reservoir solution 0.2 M magnesium chloride hexahydrate, 30%(w/v) PEG 4000, 0.1 M Tris pH 8.5
Volume and ratio of drop (µl) 3 (1.5 + 1.5)
Volume of reservoir (µl) 100