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. 2016 Jan 1;72(Pt 1):42–48. doi: 10.1107/S2053230X15023316

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well plate
Temperature (K) 277
Protein concentration (mg ml−1) 15
Buffer composition of protein solution 10 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol, 5 mM βMe
Composition of reservoir solution 12.5% PEG 3350, 12.5% PEG 1000, 12.5% MPD, 0.03 M amino-acid mix
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (ml) 0.5