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. 2016 Jan 1;72(Pt 1):49–52. doi: 10.1107/S2053230X15023274

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type 24-well VDX plates
Temperature (K) 293
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris–HCl, 10 mM Na2HPO4, 300 mM NaCl, 5 mM DTT pH 8.0
Composition of reservoir solution 2.0 M NaCl, 0.1 M sodium acetate pH 4.5
Volume and ratio of drop 1 µl, 1:1
Volume of reservoir (µl) 400