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. Author manuscript; available in PMC: 2016 Jan 11.
Published in final edited form as: FASEB J. 2008 Feb 8;22(7):2297–2310. doi: 10.1096/fj.07-099481

Figure 4.

Figure 4

VEGFR-2 levels and its phosphorylation were decreased in response to CS and/or VEGFR-2 inhibition in mouse lung. Soluble proteins from lung homogenates were electrophoresed in a 4 –15% gradient PAGE gel and electroblotted into nitro-cellulose membranes. A) p-VEGFR-2 and VEGFR-2 protein were measured using immunoblotting with rabbit anti-tyr(p)-VEGFR-2 (Clone 4G10-universal p-tyr antibody, Upstate Cell Signaling) and anti-VEGFR-2 primary antibodies (Cell Signaling), respectively. B) Histograms represent mean ± SE relative levels of p-VEGFR-2 (n=4). *P < 0.05 vs. both vehicle-treated air- and CS-exposed groups. C) Histograms represent mean ± SE relative VEGFR-2 expression in mouse lungs (n=4). *P < 0.05 vs. vehicle-treated air-exposed group.