Fig 1. Identification of neuronal IIS/FOXO targets requires neuronal isolation.

A) Volcano plot of neuron-expressed relative to whole worm-expressed genes obtained by neuron-specific RNA sequencing of adult wild-type animals. B) Neuron-expressed and enriched genes are not influenced by cell isolation: treatment with the transcription inhibitor Actinomycin D affected only 0.22% of all neuronal genes (Supplementary Table 4). C) Tissue expression prediction of wild-type adult neuron-enriched genes. Mean ± SEM. D) GO terms highlight the neuronal characteristics of both all and previously uncharacterized neuron-enriched genes. E) Embryonic¹⁶, larval¹⁶, and adult neuron-enriched genes and significant GO terms transition from developmental to neuronal and behavioral functions (Supplementary Table 5); FDR<10% for all gene sets.