Extended Data Fig 10.

Adult-only (A) or whole-life (B) fkh-9 RNAi treatment increases matricide in daf-2 worms. The cumulative % of animals dead as a result of bagging and/or exploding was recorded every other day. Two biological replicates were performed, with a representative experiment shown. C) Neuronal rescue of fkh-9 in daf-2;fkh-9 animals does not diminish the rate of vulval protrusions with age. N ≥ 60 per conditions for each experiment. D) Neuronal rescue of fkh-9 does not restore longevity of the daf-2;fkh-9 double mutant. daf-2 median lifespan: 41 days, daf-2;fkh-9 20 days, daf-2;fkh-9;Punc-119::fkh-9 20 days. p < 0.0001 for daf-2 vs. both daf-2;fkh-9 and daf-2;fkh-9;Punc-119::fkh-9. N=112 worms per strain. Censor rate for daf-2 19%, daf-2;fkh-9 51%, daf-2;fkh-9;Punc-119::fkh-9 56%.