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. Author manuscript; available in PMC: 2016 Jun 14.
Published in final edited form as: Nature. 2015 Dec 14;529(7584):92–96. doi: 10.1038/nature16483

Fig 3. FKH-9 is a direct target of DAF-16 and is expressed in mechanosensory neurons.

Fig 3

A, B) daf-16 is required for daf-2’s enhanced Day 5 axon regeneration, Mean ± SEM, *p<0.05, Fisher’s exact test. C) Known larval regeneration genes are significantly enriched in the Day 1 adult mechanosensory transcriptome. 63 genes are both DAF-16 targets and expressed in mechanosensory neurons (<5%FDR). D) fkh-9 mRNA levels are higher in aged daf-2 compared to wild type in a daf-16-dependent manner. N=4 biological replicates, two-way ANOVA, Bonferroni post hoc tests. E) Chromatin immunoprecipitation of DAF-16::GFP worms with and without heat shock, which mobilizes DAF-16 into the nucleus. DAF-16 binds to the sod-3 promoter but not its 3’ UTR, and to the fkh-9 promoter at multiple locations (Extended Data Figure 8). Fold enrichment relative to wild-type (not expressing DAF-16::GFP) is shown (mean ± SEM, two-tailed t-test, N=3 biological replicates). F) Neuronal FKH-9::GFP (fkh-9p::fkh-9::gfp) expression in daf-2 compared to wild type. N=25 animals. Mean ± SEM, two-tailed t-test. D–F) *p<0.05, **p<0.01, ***p<0.001.

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