Table 2.

[RNQ+] variants isolated from wild yeast exhibit diverse biochemical properties and aggregate distribution

Strain	Temperature sensitivity of Rnq1 aggregates	Rnq1-GFP distribution
RM9-1B	[rnq]	diffuse
YJM326	[rnq]	diffuse
YSP163a	[rnq]	diffuse
C1:0382	[rnq]	diffuse
RM5-1B	Breakdown at 65°C, gone at 95°C	SD
12447-1-3a	Breakdown at 65°C, gone at 95°C	SD
FL100	Breakdown at 65°C, gone at 95°C	SD
YJM653	Breakdown at 65°C, gone at 95°C	SD
Y-2209	Breakdown at 65°C, gone at 95°C	SD
F1643	Breakdown at 65°C, gone at 95°C	SD
T73	Breakdown at 65°C, gone at 95°C	SD and 2D
Y-399	Breakdown at 65°C, gone at 95°C	SD and 2D
F1640	Breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
YJM237	Breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
YJM431	Breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
RM7-1A	Breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
CLIB324	Breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
Y-27806	No breakdown at 65°C, gone at 95°C	SD
F1632	No breakdown at 65°C, gone at 95°C	SD
F1653	No breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
UCD939	No breakdown at 65°C, gone at 95°C	diverse: SD,2D,MD
UCD824	No breakdown at 65°C, aggregates remain at 95°C	SD

Lysates from 74-D694 yeast cells harboring the wild [RNQ⁺] variants from the yeast strains listed in column 1 were incubated at 25°C, 65°C or 95°C, and analyzed by SDD-AGE as detailed in Materials and Methods and Figure 1. Variants were categorized as listed in column 2. Rnq1-GFP was expressed in 74-D694 yeast cells harboring the wild [RNQ⁺] variants and live cells were analyzed by fluorescence microscopy. Variants were categorized according to the number of fluorescent puncta contained per cell in column 3. MD= multiple fluorescent puncta, 2D= two fluorescent puncta, SD= single fluorescent puncta. Four [rnq-] control 74-D694 strains showed only monomer when analyzed by SDD-AGE and had diffuse GFP fluorescence.