Table 1.

DNA Primers used in this study

Primer name Sequence

Universal for cDNA synthesis, RS19-dT15

5′-AGCACTGACCGATGTCACG-(T)15-3′

Primers for Q-PCR

Bax-Fw  5′-TGCTAGCAAACTGGTGCTCAA-3′

Bax-Rv  5′-AGCCCATGATGGTTCTGATCAGCT-3′

Bbc3-Fw  5′-ACCTCAACGCGCAGTACGA-3′

Bbc3-Rv  5′-TGAGGGTCGGTGTCGATGCT-3′

Cdkn1a-Fw  5′-GAGGCAGACCAGCCTGACA-3′

Cdkn1a-Rv  5′-TCTGCGCTTGGAGTGATAGAAAT-3′

Myc-Fw  5′-AGCCCCTAGTGCTGCATGA-3′

Myc-Rv  5′-GTTTGCCTCTTCTCCACAGACAC-3′

Gapdh-Fw  5′-TGGCCAAGGTCATCCATGACAAC-3′

Gapdh-Rv  5′-TCCAGAGGGGCCATCCACAGTCTTCTG-3′

Pfx(high-fidelity)-PCR primers for reference preparation

Bax-Fw  5′-TGCTGACGTGGACACGGA-3′

Bax-Rv  5′-ACAAAGATGGTCACTGTCTGCCATG-3′

Bbc3-Fw  5′-ACCACAACGCGCAGTACGA-3′

Bbc3-Rv  5′-AGTCCAGTATGCTACATGGTGCA-3′

Cdkn1a-Fw  5′-AGGCCCGAGAACGGTGGAACTTT-3′

Cdkn1a-Rv  5′-TCTGCGCTTGGAGTGATAGAAAT-3′

Myc-Fw  5′-AATCCTGTACCTCGTCCGATTC-3′

Myc-Rv  5′-ACCCTGCCACTGTCCAACT-3′

Gapdh-Fw  5′-GTCTTCACCACCATGGAGAAG-3′

Gapdh-Rv  5′-TCCAGAGGGGCCATCCACAGTCTTCTG-3′