Table 1. Bacterial strains and plasmids used in this study.
| Strains/plasmids | Relevant properties a | Source |
|---|---|---|
| Escherichia coli Trans1-T1 | F-φ80(lacZ)ΔM15ΔlacX74hsdR(rk-mk+)ΔrecA1398endA1tonA | TransGen |
| E. coli EC135/pM. Bam | E. coli EC135 harboring pM. Bam plasmid | [22] |
| Bacillus subtilis WB600 | nprE aprE epr bpr mpr::ble nprB::bsr Ʃvpr | [23] |
| Bacillus subtilis AS.1398 | Neutral protease industrial strain | This study |
| Bacillus amyloliquefaciens K11 | Wild type strain with high neutral protease-producing capacity | ACCC19735 |
| Bacillus amyloliquefaciens 110N-6 | B. amyloliquefaciens K11 harboring Bacillus sp. high copy number vector pUB110-Banpr | This study |
| Bacillus amyloliquefaciens 110N-N3 | B. amyloliquefaciens K11 harboring pUB110-Banpr vector | This study |
| pEASY-T3 | E. coli cloning vector; AmpR | TransGen |
| pUB110 | Bacillus spp. expression vector; KanR | BGSC |
| pUB110-Banpr | pUB110 harboring Banpr gene of B. amyloliquefaciens K11 | This study |
a AmpR, ampicillin resistance; KanR, kanamycin resistance