Table 4. Segregation stability of pUB110-Banpr in recombinant Bacillus amyloliquefaciens 110N-6 by continuous passage cultivationa.
No. of generation | The number of colonies in 10−5-diluted plates | The number of colonies in 10−6-diluted plates |
---|---|---|
1 | 370 ± 2 | 54 ± 1 |
10 | 400 ± 6 | 50 ± 4 |
20 | 320 ± 1 | 48 ± 2 |
30 | 410 ± 5 | 58 ± 5 |
40 | 380 ± 3 | 49 ± 1 |
50 | 340 ± 1 | 43 ± 2 |
60 | 330 ± 4 | 47 ± 1 |
70 | 310 ± 2 | 56 ± 4 |
80 | 290 ± 3 | 45 ± 3 |
90 | 280 ± 6 | 48 ± 3 |
100 | 260 ± 3 | 43 ± 1 |
a All data are shown as mean ± SD (n = 3)