Fig 4. Effect of AdcAII on in vivo colonization and invasion.

A) For colonization studies, mice were inoculated intranasally with bacteria (7x10³ CFU). At 24 and 48 hrs, mice were sacrificed and 200μL of PBS was passed from the trachea through the nose. Nasal passages were also collected, homogenized, and serial dilutions were plated. B) For invasion studies, mice were inoculated intranasally with bacteria (3.6x10⁴ CFU). Serial dilutions of blood samples were plated at 24 and 48 hrs. Results have been pooled from two independent experiments.