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. 2016 Jan 11;11(1):e0147013. doi: 10.1371/journal.pone.0147013

Fig 4. NIK knockdown attenuates MEHP-induced upregulation of CRH and COX-2 in the human placenta.

Fig 4

Primary term cytotrophoblast were incubated with siRNAs targeting NIK (siNIK) for 24 hrs, and then treated with MEHP for an additional 24 hrs. Scramble siRNA (Scr-siRNA) was used as the non-targeting control. Total RNAs were extracted and RT-qPCR was performed to determine mRNA levels of CRH (A), COX-2 (B), or NIK (C) (N = 3 independent experiments). * p < 0.05 (compared to DMSO). ** p< 0.01 (compared to DMSO). ## p < 0.01 (compared to Scr-siRNA). (D) Representative gel pattern of Western blot analysis on whole cell lysates from three independent experiments. (E) IF staining was performed to directly assess intracellular distribution of RelB. These images represent data obtained from three independent placentas. Original magnification, 200Χ.