Fig 4. D₅R and CCK_BR physical interaction in HK-2 and HEK293-D₅R-CCK_BR cells.

Co-immunoprecipitation of D₅R and CCK_BR in HK-2 cells (A) and HEK293-D₅R-CCK_BR cells (B). Whole cell lysates were subjected to immunoprecipitation (IP) with mouse anti-D₅R antibody, mouse anti-CCK_BR antibody, or non-immune mouse serum (negative control). Immunoprecipitated complexes were analyzed by immunoblotting (western blot, WB), using rabbit anti-D₅R antibody or rabbit anti-CCK_BR antibody. PC: positive control; NC: negative control. These experiments were repeated three times with similar results.