Fig 4. Cross-reactive CD4⁺ and CD8⁺ T cells obtained from Ad vector immunized mice produce cytokines upon ex vivo stimulation with various HCV proteins.

Splenocytes obtained from Ad vector immunized mice were cultured with HCV core, NS3, NS4 or NS5 antigens at 5 μg/ml, and analyzed after 5 days for intracellular IFN-γ and IL-10 expression profile of CD4⁺ and CD8⁺ T cells by flow cytometry. Data are obtained from a pool (n = 5) of spleen cells and are representative of two independent experiments.