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. 2016 Jan 11;10(1):e0004338. doi: 10.1371/journal.pntd.0004338

Fig 1. T regulatory cells in Leprosy.

Fig 1

A) Representative FACS plot showing enumeration of Treg cell frequency (CD4+, CD25+ and FoxP3+) in BT/TT vs. BL/LL patient B) Total frequency of circulating Treg (CD4+, CD25+ and FoxP3+) cells in PBMCs isolated from peripheral blood of BT/TT vs. BL/LL patients (n = 20) vs. Healthy Contacts (n = 10). C) Representative flow cytometric analysis showing IL-10 production following in vitro stimulation with M. leprae (WCL) vs. unstimulated for 48 h in a Leprosy patient with LL. D) Scatter Plots are showing total IL-10 production in gated CD4+ FoxP3+ cells in BT/TT vs. BL/LL (n = 15) vs. Healthy Contacts (n = 10). E) CCR4 surface expression on gated CD4+ FoxP3+ cells in different groups of Leprosy patients (BT/TT vs. BL/LL), (n = 10). Each dot represents a single individual. Median values are shown in each set while P value< 0.05 was considered to be significant. Total number of cells analyzed by flow cytometry were 500,000. Data analysis was performed with flowjo software. Statistical analysis was done using Student’s t test for unpaired samples.