Fig 1. UID-targeted DNA sequencing workflow and the principle in distinguishing errors from true mutation.

(A) Illustration of UID-targeted DNA sequencing workflow. (B) True mutation from errors introduced during PCR and sequencing. A true mutation (illustrated as red star) is expected to be present in all the reads carrying the same UID (or derived from the same template molecule), while an error (illustrated as blue star) is expected in some but not all the reads carrying the same UID.