Table 8. PCR sampling efficiency for each genes in multiplex PCR.
| Template # | Reads # | UID# | % Template Sampled | |
|---|---|---|---|---|
| FGFR3-E14 | 291850 | 64472 | 997 | 0.30% |
| FGFR3-E7 | 291850 | 6569826 | 104094 | 35.60% |
| FGFR3-E9 | 291850 | 57895 | 1589 | 0.50% |
| HRAS-E1 | 291850 | 133233 | 4856 | 1.70% |
| HRAS-E3 | 291850 | 111253 | 2476 | 0.80% |
| PIK3CA-E20 | 291850 | 13878 | 594 | 0.20% |
| PIK3CA-E9 | 291850 | 57895 | 2494 | 0.90% |
0.1 ng input or 291850 copies of plasmid molecules was used as templates. High fidelity Q5 enzymes with spike-in Taq DNA polymerase was used for both Stage 1 and Stage 2 amplification.