Fig 2. Glucose inhibits proliferation of cells deleted of mtDNA.

(A) Decreasing glucose concentration leads to increased proliferation of cells lacking mtDNA. Strain BY4742 (WT) was cultured in YEPD medium containing 2%, 0.5%, or 0.2% glucose and tested for the response to mtDNA deletion. Cells were incubated for 3 d. (B) Proliferation of ρ⁰ cells by Gpa2p or Gpr1p deletion is not improved further upon lowering the glucose concentration. Strains BY4742 (WT), CDD849 (gpa2Δ), and CDD850 (gpr1Δ) were treated as in (A), yet incubated on solid medium for 2 d.