Fig 5. Several transcription factors driving stress resistance following PKA inhibition are not individually responsible for the benefits provided by Pde2p overexpression to cells lacking mtDNA.

(A) Cells lacking Gis1p and mtDNA are increased in proliferation upon Pde2p overexpression. Strains BY4742 (WT) and CDD801 (gis1Δ) were treated as in Fig 1A. (B) Cells lacking both Msn2p and Msn4p exhibit increased fitness following mtDNA loss upon Pde2p overexpression. Strains BY4741 (WT) and CDD838 (msn2Δ msn4Δ) were treated as in Fig 1A. (C) The Rim15 kinase is not required in order for Pde2p overexpression to benefit ρ⁰ cells. Strains CDD463 (WT) and CDD841 (rim15Δ) were treated as in Fig 1A. (D) A potential reduction of Hsf1p function does not prevent increased ρ⁰ cell fitness upon overexpression of Pde2p. Strains BY4741 (WT) and CDD910 (hsf1-DAmP) were treated as in Fig 1A.