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. Author manuscript; available in PMC: 2016 Dec 21.

Published in final edited form as: Dev Cell. 2015 Dec 21;35(6):737–749. doi: 10.1016/j.devcel.2015.11.027

A) Q ratio image of wing tissue expressing the CWT sensor and containing clones of ds⁻ft⁻ cells (outlined; Q ratio scale as in Fig. 2). Clones appear red relative to the wildtype surround. See Fig. S3 for Q ratio images of tissue containing single mutant ds⁻ and ft⁻ clones, and Fig. S4 for further examples and documentation of Q ratios and CWT abundance associated with ds⁻ft⁻ cells relative to surrounding wildtype cells.

As diagrammed underneath, Q (the ratio of acceptor and donor channel emissions) is expected to rise ( ) if the mutant condition causes an increase in either energy transfer or C-terminal proteolysis.

B) Q ratio image for ds⁻ft⁻ clones in wing tissue expressing the TWC sensor. As in A, clones appear red relative to the surround, as expected if energy transfer increases in the mutant cells ( ). However, clones should appear blue in the case of C-terminal proteolysis, which was not observed ( ; greyed out). Note that the difference between the clone and surround is less pronounced for the TWC than the CWT sensor (A), possibly reflecting a less favorable dipole orientation of the two fluorophores in the TWC sensor.

Inline graphic — A) Q ratio image of wing tissue expressing the CWT sensor and containing clones of ds⁻ft⁻ cells (outlined; Q ratio scale as in Fig. 2). Clones appear red relative to the wildtype surround. See Fig. S3 for Q ratio images of tissue containing single mutant ds⁻ and ft⁻ clones, and Fig. S4 for further examples and documentation of Q ratios and CWT abundance associated with ds⁻ft⁻ cells relative to surrounding wildtype cells.

As diagrammed underneath, Q (the ratio of acceptor and donor channel emissions) is expected to rise ( ) if the mutant condition causes an increase in either energy transfer or C-terminal proteolysis.

B) Q ratio image for ds⁻ft⁻ clones in wing tissue expressing the TWC sensor. As in A, clones appear red relative to the surround, as expected if energy transfer increases in the mutant cells ( ). However, clones should appear blue in the case of C-terminal proteolysis, which was not observed ( ; greyed out). Note that the difference between the clone and surround is less pronounced for the TWC than the CWT sensor (A), possibly reflecting a less favorable dipole orientation of the two fluorophores in the TWC sensor.