Fig 8. hWH knockdown results in DNA damage at the replication sites.

(A) HCT116 p53^+/+ cells were treated with either hWH or control siRNA for 24, 48, and 72 h. Afterward cells are harvested and stained with DAPI (blue), EdU (green), and γ-H2AX (red). (B) After siRNA treatment for 48h, γ-H2AX positive cells percentage in sihWH is significantly higher than that of siControl. Double asterisks indicate significant differences at p < 0.01, according to Student’s t test.