Fig 4. Rv1410 function is necessary for growth of Mtb in mice with immune deficiencies, indicating that loss of virulence is intrinsic to the bacterium.

Colony forming units (cfu) of WT (H37Rv, black) and rv1410::Tn (Mut1, red) from spleen and lungs of mice infected with a 3:1 (Mut1:WT) mixture. (A) C57/Bl6 (B) nos2-/- (C) phox-/- (D) phox-/- fed aminoguanidine ad libitum, and (E) inf-γ -/- mice. Cfu were measured in lung and spleen at 1, 7, and 30 days post infection. Error bars show mean +/- SD of three to five animals per time point.