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. 2016 Jan 11;12(1):e1005351. doi: 10.1371/journal.ppat.1005351

Fig 6. Carbon restriction slows growth of LprG-Rv1410 mutants in vitro and phenocopies slow growth in vivo.

Fig 6

WT, Mut2, and Comp2 were cultured in (A) 7H9 plus ADC (albumin, dextrose, catalase) supplement, 0.2% glycerol, and 0.05% Tween80; (B) Sauton’s minimal medium plus 0.01% cholesterol, 0.05% Tyloxapol and with (dashed) or without (solid) tetrahydrolipostatin (THL; 5 μg/mL); (C) Sauton’s minimal medium, 10mM propionate; (D) 7H9 with ADC, 0.05% Tween80, 10 mM propionate with (large dashed) or without (solid) 5 mM acetate; (E) Sauton’s minimal medium plus 0.05% Tyloxapol, 10mM propionate with (large dashed) or without (solid) 5 mM acetate; (F) 7H9 plus ADC, 0.2% glycerol, 10 mM propionate with (large dashed) or without (solid) 5 mM acetate; (G) 7H9 plus ADC with 10 mM propionate, 5 mM acetate, and 0.05% oleic acid (small dashed). (F,G) represent one experiment. Growth was measured as OD600 at the indicated times. Generation time during exponential growth was estimated from the log-linear slope (S2 Table). Data are the average of three technical replicates and are representative of three independent experiments. Error bars (within data symbols) are +/- SD. Mut2+ LprG (ΔlprG-rv1410c L5::lprG); Mut2+ Rv1410 (ΔlprG-rv1410c L5::rv1410c).