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. 2016 Jan 11;11(1):e0146320. doi: 10.1371/journal.pone.0146320

Fig 1. Interactions of candidate clones and the CMV-P1 RNA1 helicase domain using in the yeast two-hybrid.

Fig 1

The bait was the CMV-P1 RNA1 helicase domain. For prey, PMM, Phosphomannomutase; FDH, Formate dehydrogenase; CRT3, Calreticulin-3 precursor; UBI11, Polyubiquitin 6PU11; CysK, Cysteine synthase; AGPase, ADP-glucose pyrophosphorylase; ARF1, ADP-ribosylation factor 1; ARF, ADP-ribosylation factor; H3, Histone-H3; ARD, Acireductone dioxygenase were used. P and N represent positive and negative controls, respectively. Empty is a bait vector alone. SD medium (-LW; lacking tryptophan and leucine and -LWH; lacking tryptophan, leucine, and histidine) was used to select for co-transformation. β-galactosidase (β-Gal) activity assays were performed according to the manufacturer’s protocol.