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. 2016 Jan 11;11(1):e0146320. doi: 10.1371/journal.pone.0146320

Fig 5. CMV accumulation in FDH- and CRT3-silenced N. benthamiana.

Fig 5

(a) CMV accumulation in plants silenced for FDH and CRT3 genes. Virus accumulation was detected by ELISA. Two leaf discs of the inoculated and upper leaves of the inoculated plants were sampled at 11 dpi. Means and standard deviations were obtained from three biological replicates. (b) The mRNA expression levels of FDH and CRT3 were evaluated by real-time PCR analysis at 11 dpi. To normalize the expression level, N. benthamiana Actin transcript was used. Two leaf discs of the inoculated leaves were sampled at 11 dpi. Empty vector was the positive control. #1, #2, and #3 represent biological replicates. Asterisks indicate statistically significant differences relative to the empty vector as determined by Student’s t-test (*P< 0.05).