Table 3. List of forward and reverse primers and PCR conditions.

Gene	Primer	Sequence	PCR conditions
18S	Forward Reverse	CTTAGAGGGACAAGTGGCG ACGCTGAGCCAGTCAGTGTA	20s@95°C, 30s@60°C, 30s@72°C x 30 cycles
Kc	Forward Reverse	CGCTGCTGCTGCTGGCCACCA GGCTATGACTTCGGTTTGGGTGCA	20s@95°C, 30s@60°C, 30s@72°C x 60 cycles
Gmcsf	Forward Reverse	TGGTCTACAGCCTCTCAGCA GCATGTCATCCAGGAGGTTC	20s@95°C, 30s@60°C, 30s@72°C x 60 cycles
Ifng	Forward Reverse	GTGGTTGACACTTAGTGGTCTC GGTGACATGAAAATCCTGCAGAGC	20s@95°C, 30s@60°C, 30s@72°C x 60 cycles
Tnfa	Forward Reverse	AGTTCTATGGCCCAGACCCT AGGGTCTGGGCCATAGAACT	20s@95°C, 30s@62°C, 30s@72°C x 60 cycles
Mif	Forward Reverse	CAAGCCCGCACAGTACATC AGGCCACACAGCAGCTTACT	20s@95°C, 30s@60°C, 30s@72°C x 60 cycles
Mif (Cig Smoke)	Forward Reverse	CGTGCACTGCGATGTACTGT CCATGCCTATGTTCATCGTG	20s@95°C, 30s@60°C, 30s@72°C x 60 cycles
Hprt	Forward Reverse	AGGCCAGACTTTGTTGGATTTGAA CAACTTGCGCTCATCTTAGGCTTT	20s@95°C, 30s@60°C, 30s@72°C x 60 cycles