Figure 3. SAS-6 self-assembly is not essential for the ninefold symmetry of centrioles.
Isogenic SAS-6-/-; p53-/- cells carrying indicated SAS-6 constructs (see ‘Methods’) were induced to express indicated SAS-6 mutants for 3 days, and then processed for serial sectioning electron microscopy. (A,B) Mature canonical centrioles in WT RPE1 cells, or de novo centrioles formed in SAS6-/- cells expressing full-length (FL) or mutant SAS-6 as indicated are shown in longitudinal (A) or cross sectional (B) views. Note that these centrioles were mature and have acquired appendages. (C) Ciliated centrioles from indicated cell types were serially sectioned and examined by EM. (D) Duplicated, engaged centriole pairs from indicated cell types were serially sectioned and examined by EM. Scale bar: 200 nm.