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. 2015 Dec 24;14(1):152–167. doi: 10.1016/j.celrep.2015.12.020

Figure 1.

Figure 1

Generation of Conditional and Constitutive E-cadherin-GFP Mice

(A) Schematic of the generation of a conditional E-cadherin-GFP OFF strain by targeting a lox-stop-lox transgene under the control of a CAG promoter to the Hprt locus.

(B) Conditional E-cadherin-GFP expression in pancreas (Pdx1-Cre), liver (Cyp1a1-Cre), mammary gland (MMTV-Cre), and pancreatic islets (RIP-Cre). Shown are western Blot analyses in wild-type (WT) and transgenic organs (top band, E-cadherin-GFP; bottom band, endogenous E-cadherin) and corresponding multiphoton images.

(C and D) Western Blot analyses in 16 organs (C) and multiphoton images of E-cadherin GFP expression (D, column 1) and confocal FRAP (D, columns 2–4) in six organs of the E-cadherin-GFP ON mouse. E-cadherin-GFP, green; SHG signal, purple; red arrow, bleach point.

See also Figure S1.