Figure 3.

FRAP and FLIP in Live Tumors Reveal E-cadherin Mobilization during Invasion

(A) Representative images of encapsulated p53^−/− vector tumors (left, dashed line, black arrows) versus locally invasive p53^−/− R175H tumors (right, white arrows).

(B) Representative images from confocal FRAP movies of p53^−/− vector (top) versus p53^−/− R175H xenografts (bottom). Red arrows, bleached regions.

(C and D) FRAP recovery curves (C) and mobile fraction plots (D) comparing E-cadherin-GFP mobilization between p53^−/− vector and p53^−/− R175H cells grown in a 2D versus 3D environment. n = 3, >10 junctions/group.

(E) Representative images from FLIP movies of p53^−/− vector (top) and p53^−/− R175H xenografts (bottom). White rectangles, bleach regions; red lines, tracked bleached junctions.

(F) Photobleach-corrected kymographs showing the intensity profile along the bleached junction over time. Arrows, bleach events.

(G and H) Photobleach-corrected, normalized intensity profiles (G) and averaged fraction of fluorescence retained after 400 s (H) for the bleached region (red), areas 3 μm distant from the bleached region (blue), and distant control junctions (orange) of p53^−/− vector (light colors, n = 9) and p53^−/− R175H cells (dark colors, n = 5).

Columns, mean; bars, ± SE; n = 3 mice; >5 cells/group. ^∗p < 0.05; ^∗∗p < 0.01 (unpaired Student’s t test). See also Figures S2 and S3.