Table 1.
Description of candidate reference genes and MAR in P. ginseng.
| Gene symbol | Gene description | GenBank accession number | Arabidopsis ortholog | Arabidopsis BlastX E-value | Primer sequences (5′–3′) (forward/reverse) | Primer Tm (°C)a | Product (bp) | Product Tm (°C)b | E(%)c | R2c |
|---|---|---|---|---|---|---|---|---|---|---|
| REFERENCE GENES | ||||||||||
| ACT11 | Actin 11 | KU215665 | NP_187818.1 | 0 | CCCGAGAGAAAGTATAGTGTATGGA | 59.91 | 171 | 81.5 | 92.6 | 0.998 |
| TAGAGCTCTTCAACAACCACTTTTT | ||||||||||
| ACT | Actin | KU215664 | AAO42312.1 | 1e-55 | AAAGATTTGGCATCACACCTTCTAC | 60.95 | 203 | 83 | 111.4 | 0.990 |
| TACCTGTTGTACGACCACTAGCATA | ||||||||||
| EF1-β | Elongation factor 1-beta | KU215660 | NP_179402.1 | 1e-44 | AAGAAGAAAGAGAGTGGGAAATCAT | 60.02 | 158 | 82 | 112.5 | 0.997 |
| TTTATCCCATAACCAACAGGAAGTA | ||||||||||
| EF1-γ | Elongation factor 1-gamma | KU215661 | NP_176084.1 | 1e-60 | ATCGCATTAAAGAGAGCACTAGGA | 60.11 | 186 | 80.5 | 105.5 | 0.997 |
| CATGGTCCAAAAATATCTCTCTACG | ||||||||||
| IF3G1 | Eukaryotic translation initiation factor 3G1 | KU215663 | AAG53636.1 | 3e-34 | GCGAACTAGAATATTTTCATCTCCA | 59.90 | 208 | 79 | 113.9 | 0.994 |
| AGAAGAGAAGGGATAACAATTCCAT | ||||||||||
| IF3B | Eukaryotic translation initiation factor 3B | KU215662 | NP_568498.1 | 9e-109 | CCTTGGTTACTGCTTCATTGAGTAT | 59.94 | 198 | 81 | 110.2 | 0.997 |
| TGTTGTAAATTTTCCCCTGATGTAT | ||||||||||
| GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | AY345228 | NP_172801.1 | 0 | AAGGTTAAGGACAATAACACCCTTC | 60.06 | 195 | 76.5 | 88.9 | 0.994 |
| AGGAGCTGAAATAACAACCTTCTTT | ||||||||||
| CYC | Cyclophilin ABH-like protein | EF587913 | NP_179251.1 | 2e-100 | ATCTGAGTCTGGGTTGTGATAAAAG | 60.00 | 175 | 85.5 | 117.3 | 0.994 |
| AACAACCAACCAAACCAAATTATTA | ||||||||||
| GENE | ||||||||||
| MAR | Multiple-function-associated mlncRNA | KU285606 | N | N | GATGGTCCAGAGGATAGCATTAGTA | 59.98 | 223 | 77.5 | 100.7 | 0.997 |
| TTATACATCACCTATCTTGGGTCGT | ||||||||||
a
The primer melting temperature was calculated by Primer3 (v. 0.4.0).
b
The product melting temperature was calculated by CFX Manager™ software.
c
The RT-qPCR efficiency (E) and correlation coefficients (R2) were determined by CFX Manager™ software.
N, Not examined.