Figure 5.

Effects of iodothyronines on triglyceride secretion. (A) The extracellular TAG content was quantified in the culture medium in control and “steatotic” cells incubated in the absence (NEFA) or in the presence of T₂ or T₃ (10⁻⁵M, 24 h). The mRNA (B) levels of ApoB 100 were measured by qPCR, in control and “steatotic” cells incubated in the absence (NEFA) or in the presence of T₂ or T₃ (10⁻⁵ M, 24 h). GAPDH was used as the internal control for quantifying gene expression. Data, expressed with respect to controls, are the mean ± S.D. of at least four experiments in triplicate. Significant differences are denoted by symbols on bars (C vs. NEFA ^*p ≤ 0.05, ^**p ≤ 0.01).