Figure 3. ISC tumor initiation and outgrowth requires autonomous Spi/EGFR signaling.

(a–c) Phosphorylated histone H3 Ser10 (red) and GFP (green) after expression of N^RNAi (a), EGFR^RNAi and N^RNAi (b) and spi^RNAi and N^RNAi (c) with esg^ts for 3 days.

(d) Phosphorylated histone H3 Ser10 (red) and GFP (green) after expression of spi^RNAi and N^RNAi with esg^ts for 14 days.

(e) Mean number of phosphorylated histone H3 Ser10 positive cells per midgut with s.e.m. after expression with esg^ts of N^RNAi and GFP (n=30 midguts), N^RNAi and Egfr^RNAi (n=30 midguts, Mann-Whitney: p=0.0001), N^RNAi and spi^RNAi for 3 (n=33 midguts, Mann-Whitney: p<0.0001) or 30 days, and N^RNAi and Mkp3 for 5 or 10 days. Midguts were pooled from 3 independent experiments.

(f) Survival after expression of GFP alone (control), spi^RNAi, N^RNAi, and N^RNAi and spi^RNAi with esg^ts (n=100 flies per genotype pooled from 2 independent experiments).

DNA is a-d (blue). Scale bars in a–c, 40 µm; d, 50µm.