Figure 4. cGAMP Triggers Dendritic Cells Activation.

Colon 26 tumor-bearing mice were treated with cGAMP from day 0 to day 20 with 20 mg/kg once a day. Flow cytometry was applied to detect DCs from spleen with (A) isotype control, (B) key surface markers: CD40⁺, CD80⁺, CD86⁺ and MHC-II⁺ on 14^th day and (C) 20^th day, respectively, and calculated with Mean Fluorescence Intensity (MFI) (D,E). Representative data are shown from three experiments conducted with 10 mice per group. Data are represented as mean ± SEM, **p < 0.01 (Student’s t test in D and E).