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. 2016 Jan 12;6:19104. doi: 10.1038/srep19104

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Copyright © 2016, Macmillan Publishers Limited

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(a) PCR analysis of transgenic T₀ indica generation plants. M, Trans2K Plus DNA Marker; lane 1, untransformed MH86; lane 2 to 29, represents transgenic events P1-P28. (b) expression of aroA_{J. sp} cDNA in transgenic rice plants. Upper, Lanes 1 to 22, represents transgenic plants PP1-PP22, N, the non-transgenic rice control plant. Nether, the actin gene amplified from all the tested samples. (c) Southern blot analysis of transgenic T₀ indica plants of cv. MH86 with Hind III digestion. N, untransformed MH86; lane 1–15, different transgenic lines, corresponding sample numbers were marked. (d) Southern blot analysis of transgenic T₂ indica generation plants with SacI digestion. Lane 1, untrasformed MH86; lane 2–5, confirmed single copy, corresponding sample numbers were marked. (e) glyphosate tolernace of T₂ transgenic rice plants under different glyphosate dosages. T₂ rice seeds were germinated in field and grown to tillering stage. 0 g/ha, 210 g/ha, 420 g/ha, 840 g/ha, 1680 g/ha and 3360 g/ha glyphosate (the corresponding applied concentration of glyphosate to each dosage was 0 mg/L, 187.5 mg/L, 375 mg/L, 750 mg/L, 1500 mg/L and 3000 mg/L) were sprayed on ME1 and ME2 transgenic lines along with non-transformed control plant and photograph was taken 1 week after spray. ME1-P and ME2-P represent the homozygous transgenic lines of ME1 and ME2, while ME1-N and ME2-N are their corresponding negative transgenic lines. (f) glyphosate tolerance test of T₄ generation seedlings of ME1 line. The 28-day-old ME1 T₄ generation seedlings were transplanted in the field with weeds and sprayed with 840 g/ha glyphosate (the corresponding applied concentration of glyphosate was 840 mg/L). Photographs were taken by Y.C. at day 0, 7 and 15 after spray. (g) Selection of homozygous line by glyphosate. Homozyous (HO), heterzygous (HE) T₂ transgenic rice plants along with untransformed control plants (N) were sprayed with 1% (vol/vol) solution of the herbicide Roundup containing 41% isopropylamine salt of glyphosate, and photograph was taken by Y.C. at 3 d after spray.