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. 2016 Jan 12;6:18379. doi: 10.1038/srep18379

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(a) Reducing SDS-PAGE autoradiogram analysis after click reactions of flagellin with HBc VLPs. The molar ratios of flagellin (52.7 kDa) to HBc monomer (16.7 kDa) in the reaction mixture were respectively 1:2, 1:5, 1:10, 1:20, and 1:40. (b) Estimated flagellin numbers attached to each VLP were determined by gel densitometry. (c) Bioactivity analysis of flagellin-HBc VLP conjugates produced using the 1:2, 1:10, and 1:40 reactant ratios. The data were measured in triplicate; error bars indicate the standard error of the mean (SEM).