Figure 3. Inhibition of miRNAs predicted to target HO-1 and being downregulated in ammonia-exposed astrocytes elevate HO-1 mRNA and protein levels.

Cultured astrocytes were transfected either with inhibitors specifically targeting the indicated miRNA species or without inhibitor (control) before total RNA (A) or protein (B) was isolated. (A) Validation of miRNA inhibition by inhibitors specifically targeting the indicated miRNA species using miQPCR and effect of miRNA inhibition on (A) HO-1 mRNA and (B) protein levels as analysed by realtime-PCR and Western-blot, respectively. *statistically significantly different compared to control-transfected astrocytes. Data are represented as log₂ ratios from 5–9 independent experiments.