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. 2016 Jan 12;6:18493. doi: 10.1038/srep18493

Figure 4. Inhibition of HO-1 mRNA targeting miRNAs impairs astrocyte proliferation in a HO-1-dependent way.

Figure 4

Cultured rat astrocytes were transfected with either miRNA inhibitors targeting rno-miR-221-3p, rno-221-5p, 222-3p or -326-3p or without inhibitor (control) and DNA content was quantified 48 h after seeding of the cells by fluorimetric detection of Hoechst34580 fluorescence as described in materials and methods. Fluorescence intensities measured in miRNA inhibitor-treated astrocytes are given relative to transfection control. (A) Effect of miRNA inhibition on astrocyte proliferation. (B) Effect of HO-1 inhibition by tin protoporphyin IX (SnPP, 10 μmol/l) on proliferation in miRNA inhibitor-treated astrocytes. *statistically significantly different compared to transfection controls. n.s.: not statistically significantly different as compared to SnPP-treated astrocytes. Data are from 3–4 independent experiments.