Table 2. Primer sequences used for miQPCR analysis.
Name | Primer sequence | Tm (°C) |
---|---|---|
rno-miR-221-5P | GGCATACAATGTAGATTTCG | 51.80 |
rno-miR-222-3P | CTACATCTGGCTACTGGGTG | 54.24 |
rno-miR-135a-5p | TGGCTTTTTATTCCTATGTGAG | 54.81 |
rno-miR-26a-5p | AAGTAATCCAGGATAGGCTG | 52.11 |
rno-miR-425-5p | CGATCACTCCCGTTGAG | 54.12 |
rno-miR-30e-5p | AACATCCTTGACTGGAAGG | 53.40 |
rno-miR-652-3p | CGCCACTAGGGTTGTGG | 56.90 |
rno-miR-135b-5p | GGCTTTTCATTCCTATGTGAG | 55.00 |
rno-miR-31a-5p | GATGCTGGCATAGCTGG | 54.47 |
rno-miR-125a-5p | TGAGACCCTTTAACCTGTGAG | 55.41 |
rno-miR-193-3p | GCCTACAAAGTCCCAGTG | 52.31 |
rno-miR-221-3p | TTGTCTGCTGGGTTTCG | 54.91 |
rno-miR-30b-5p | TAAACATCCTACACTCAGCTG | 51.63 |
rno-miR-326-3P | GGCCCTTCCTCCAGTG | 55.70 |
rno-miR-145-5P | TTCCCAGGAATCCCTG | 53.19 |
rno-miR-365-3p | GCCCCTAAAAATCCTTATG | 52.52 |
Upm2A | CCCAGTTATGGCCGTTTA | 55.50 |
List of miRNA specific qPCR primers and of the Universal reverse primer (Upm2A) used in this study. Primer sequences are given in 5′ to 3′ direction.