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. 2016 Jan 12;6:18493. doi: 10.1038/srep18493

Table 2. Primer sequences used for miQPCR analysis.

Name Primer sequence Tm (°C)
rno-miR-221-5P GGCATACAATGTAGATTTCG 51.80
rno-miR-222-3P CTACATCTGGCTACTGGGTG 54.24
rno-miR-135a-5p TGGCTTTTTATTCCTATGTGAG 54.81
rno-miR-26a-5p AAGTAATCCAGGATAGGCTG 52.11
rno-miR-425-5p CGATCACTCCCGTTGAG 54.12
rno-miR-30e-5p AACATCCTTGACTGGAAGG 53.40
rno-miR-652-3p CGCCACTAGGGTTGTGG 56.90
rno-miR-135b-5p GGCTTTTCATTCCTATGTGAG 55.00
rno-miR-31a-5p GATGCTGGCATAGCTGG 54.47
rno-miR-125a-5p TGAGACCCTTTAACCTGTGAG 55.41
rno-miR-193-3p GCCTACAAAGTCCCAGTG 52.31
rno-miR-221-3p TTGTCTGCTGGGTTTCG 54.91
rno-miR-30b-5p TAAACATCCTACACTCAGCTG 51.63
rno-miR-326-3P GGCCCTTCCTCCAGTG 55.70
rno-miR-145-5P TTCCCAGGAATCCCTG 53.19
rno-miR-365-3p GCCCCTAAAAATCCTTATG 52.52
Upm2A CCCAGTTATGGCCGTTTA 55.50

List of miRNA specific qPCR primers and of the Universal reverse primer (Upm2A) used in this study. Primer sequences are given in 5′ to 3′ direction.