Table 2.
miRNA oligo-DNA sequence of human heparanase gene.
| Oligonucleotide | Sequences of miRNA oligo | Corresponding sequence of mRNA |
|---|---|---|
| pmiR-HPSE-1-F | 5′-TGCTGTATCCTGGTTGACTTGAGATTGTTTTGGCCACTGACTGACAATCTCAACAACCAGGATA-3′ | 356–376 bp |
| pmiR-HPSE-1-R | 5′-CCTGTATCCTGGTTGTTGAGATTGTCAGTCAGTGGCCAAAACAATCTCAAGTCAACCAGGATAC-3′ | |
|
| ||
| pmiR-HPSE-2-F | 5′-TGCTGTACAGAGCTTCTTGAGTAGGTGTTTTGGCCACTGACTGACACCTACTCGAAGCTCTGTA-3′ | 490–570 bp |
| pmiR-HPSE-2-R | 5′-CCTGTACAGAGCTTCGAGTAGGTGTCAGTCAGTGGCCAAAACACCTACTCAAGAAGCTCTGTAC-3′ | |
|
| ||
| pmiR-HPSE-3-F | 5′-TGCTGTATACTCGAAGCTTCCTTCTCGTTTTGGCCACTGACTGACGAGAAGGACTTCGAGTATA-3′ | 1280–1301 bp |
| pmiR-HPSE-3-R | 5′-CCTGTATACTCGAAGTCCTTCTCGTCAGTCAGTGGCCAAAACGAGAAGGAAGCTTCGAGTATAC-3′ | |
|
| ||
| pmiR-HPSE-4-F | 5′-TGCTGAAAGCTGGCAAGCCCAGTGAAGTTTTGGCCACTGACTGACTTCACTGGTTGCCAGCTTT-3′ | 1560–1580 bp |
| pmiR-HPSE-4-R | 5′-CCTGAAAGCTGGCAACCAGTGAAGTCAGTCAGTGGCCAAAACTTCACTGGGCTTGCCAGCTTTC-3′ | |