Table 1.
| Spot | Protein name | Gene product | (HG/NG∗) | Theoretical | Observed | IPA network | Percent coverage | ||
|---|---|---|---|---|---|---|---|---|---|
| M r | pI | M r | pI | ||||||
| 1 | Not identified | 1.23 | |||||||
| 2 | 10 kDa heat-shock protein, mitochondrial | CH10_RAT | 2.37 | 10895 | 8.9 | 8000 | 8.3 | n/a | 77 |
| 3 | Calpactin I light chain | S10AA_RAT | 1.37 | 11182 | 6.3 | 8000 | 7.4 | 1 | 45 |
| 4 | Macrophage migration inhibitory factor | MIF_RAT | 1.34 | 12640 | 6.8 | 9000 | 7.8 | 3 | 26 |
| 5 | Cofilin-1 | COF1_RAT | 1.5 | 18749 | 8.2 | 9000 | 5.3 | n/a | 34 |
| 6 | Not identified | 1.70 | |||||||
| 7 | Not identified | 1.24 | |||||||
| 8 | Profilin-1 | PROF1_RAT | 1.34 | 15119 | 8.5 | 15000 | 8.4 | 1 | 45 |
| 9 | Cystatin B | CYTB_RAT | 0.82 | 11303 | 5.9 | 10000 | 6.5 | 2 | 80 |
| 10 | Not identified | 0.66 | |||||||
| 11 | Coactosin-like protein | COTL1_MOUSE | 1.74 | 16048 | 5.3 | 16000 | 5.5 | 1, 2 | 37 |
| 12 | Galectin-1 | LEG1_RAT | 1.23 | 15189 | 5.1 | 15000 | 5.3 | 3 | 61 |
| 13 | Histidine triad nucleotide-binding protein 1 | HINT1_MOUSE | 1.39 | 13882 | 6.4 | 11000 | 7.3 | 3 | 49 |
| 14 | 40S ribosomal protein S12 | RS12_RAT | 1.34 | 14858 | 6.8 | 15000 | 7.2 | 2 | 44 |
| 15 | Nucleoside diphosphate kinase A (NDK A) | NDKA_RAT | 1.68 | 17296 | 6.0 | 16000 | 6.6 | 3 | 39 |
| 16 | Nucleoside diphosphate kinase B | NDKB_RAT | 1.26 | 17386 | 6.9 | 18000 | 7.6 | 1 | 66 |
| 17 | Not identified | 1.89 | |||||||
| 18 | Eukaryotic translation initiation factor 5A | IF5A1_RAT | 1.23 | 17049 | 5.1 | 20000 | 5.6 | 2 | 43 |
| MIR-interacting saposin-like protein | MSAP_MOUSE | 21096 | 5.0 | 20000 | 5.6 | n/a | 29 | ||
| 19 | Not identified | 1.64 | |||||||
| 20 | Cofilin-1 | COF1_RAT | 1.63 | 18749 | 8.2 | 21000 | 8 | 1 | 65 |
| 21 | Cofilin-1 | COF1_RAT | 1.59 | 18749 | 8.2 | 21000 | 7.6 | 1 | 60 |
| 22 | Cofilin-1 | COF1_RAT | 1.57 | 18749 | 8.2 | 21000 | 7 | 1 | 54 |
| 23 | Not identified | 1.61 | |||||||
| 24 | Proteasome subunit alpha type 1 | PSA2_RAT | 1.54 | 26024 | 6.9 | 23000 | 7.5 | 1 | 47 |
| 25 | Heat-shock protein beta-1 | HSPB1_RAT | 2.31 | 22936 | 6.1 | 23000 | 6.1 | 1 | 39 |
| Phosphoserine phosphatase | SERB_RAT | 25180 | 5.5 | 23000 | 6.1 | n/a | 33 | ||
| 26 | 14-3-3 protein epsilon | 1433E_RAT | 1.37 | 29326 | 4.6 | 30000 | 4.8 | 1 | 29 |
| 27 | Proteasome subunit alpha type 2 | PSA1_RAT | 1.78 | 29784 | 6.2 | 31000 | 7.2 | 1 | 36 |
| 28 | Prohibitin | PHB_RAT | 2.24 | 29859 | 5.6 | 32000 | 6.1 | 2 | 62 |
| 29 | Not identified | 2.33 | |||||||
| 30 | Proliferating cell nuclear antigen | PCNA_RAT | 1.46 | 29072 | 4.6 | 34000 | 4.9 | 1 | 31 |
| 31 | Heat-shock protein beta-1 | HSPB1_MOUSE | 0.70 | 23057 | 6.1 | 35000 | 6.3 | n/a | 25 |
| 32 | Annexin A2 | ANXA2_RAT | 0.83 | 38939 | 7.6 | 40000 | 7.8 | 1 | 56 |
| 33 | Reticulocalbin 3 precursor | RCN3_HUMAN | 1.80 | 37470 | 4.7 | 41000 | 4.9 | 2 | 20 |
| 34 | Macrophage capping protein | CAPG_RAT | 1.53 | 39060 | 6.1 | 41000 | 6.9 | 3 | 20 |
| 35 | Acetyl-CoA acetyltransferase, cytosolic | THIC_RAT | 2.15 | 41538 | 6.9 | 41000 | 7.7 | 3 | 28 |
| 36 | SUMO-activating enzyme subunit 1 | SAE1_RAT | 2.15 | 38945 | 5.0 | 41000 | 5.4 | 2 | 54 |
| 37 | Actin, cytoplasmic-1 (beta-actin) | ACTB_RAT | 0.58 | 42052 | 5.3 | 42000 | 5.7 | 1 | 30 |
| Actin, cytoplasmic-2 (gamma-actin) | ACTG_RAT | 42108 | 5.3 | 42000 | 5.7 | 1 | 30 | ||
| 38 | Not identified | 0.6 | |||||||
| 39 | Actin-like protein 3 | ARP3_MOUSE | 0.53 | 47783 | 5.6 | 50000 | 6.5 | 1 | 38 |
| 40 | Enolase 1 | ENOA_RAT | 0.60 | 47440 | 6.2 | 52000 | 6.7 | 1 | 38 |
| RAB GDP dissociation inhibitor beta | GDIB_RAT | 51018 | 5.9 | 52000 | 6.7 | n/a | 34 | ||
| 41 | Not identified | 0.37 | |||||||
| 42 | Not identified | 0.71 | |||||||
| 43 | Protein disulfide-isomerase A3 (ERp57) | PDIA3_RAT | 0.78 | 57044 | 5.9 | 58000 | 6.4 | 3 | 50 |
| 44 | GRP58 | HNRPK_RAT | 0.68 | 51230 | 5.4 | 57000 | 6.3 | 1 | 31 |
| 45 | Not identified | 2.21 | |||||||
| 46 | Heterogeneous nuclear ribonucleoprotein L | HNRPL_MOUSE | 1.40 | 60712 | 6.7 | 58000 | 7.8 | 2 | 25 |
| 47 | T-complex protein 1, epsilon subunit | TCPE_RAT | 0.78 | 59955 | 5.5 | 58000 | 6.3 | 1 | 34 |
| 48 | Hsc70/Hsp90-organizing protein | STIP1_RAT | 1.16 | 63158 | 6.4 | 65000 | 7.2 | 1 | 38 |
| 49 | GRP 75 | GRP75_RAT | 0.82 | 74097 | 6.0 | 75000 | 6.1 | 1 | 31 |
| 50 | Not identified | 1.35 | |||||||
| 51 | RAB 6 interacting protein 2 (ERC protein 1) | GANAB_MOUSE | 0.68 | 107300 | 5.7 | 116000 | 6.4 | 2 | 21 |
Murine GMC protein expression at 2 h culture HG versus 2 h culture NG∗.