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. 2016 Jan 12;6:19226. doi: 10.1038/srep19226

Figure 13. Immunohistochemical and western blot analysis of tumor cells from the liver of ALV-J-infected chickens.

Figure 13

(a) Fresh tissues were collected and fixed in 10% neutralized buffered formalin, dehydrated, embedded in paraffin wax, and then sliced into 6-μm sections. These sections were routinely stained with hematoxylin and eosin (HE), and examined microscopically. Tissues were further immunohistochemically labeled for gp85, ERK or p-ERK with JE9 monoclonal, anti-p44/42 MAPK or anti-phospho-p44/42 MAPK (Thr202/Tyr204) antibodies, respectively. Bar = 100 μm. (b) Western blot analysis detected high expression of p-ERK proteins in myeloid leukosis (ML) samples, but not in the normal samples. Sample identification numbers are indicated above the panel as follows: T, tumor samples; C, non-tumor samples.