Figure 7. ALV infection activates ERK2 phosphorylation in a MEK-dependent manner.

DF-1 cells were preincubated with PD98059 (2.5–50 μM), U0126 (5–10 μM) or DMSO (0.1%, v/v) for 1 h and subsequently infected with CHN06 (a,b), GD13-1 (d) or CD08 (e) for 15 min, or with NX0101 (c) for 120 h, at 10⁴ TCID₅₀ 0.2 ml⁻¹. The inhibitors or DMSO were in the cell culture fluid all the time. And the concentration of inhibitor control is the maximum concentration used in each independent experiment. Cell lysates were collected and the amounts of p-ERK2 and total ERK were evaluated by western blotting. The results are representative of two independent experiments. Abbreviations: CHN, CHN06 or HN06; NX, NX0101; GD13-1, GD; CD08, CD.