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. 2016 Jan 12;6:19183. doi: 10.1038/srep19183

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(a) Genetic organization for the construction of a set of plasmids. (b) SDS-PAGE of a cell-free extract of each transformant. 1, marker; 2, wild-type NHase; 3, NHase-(BA); 4, NHase-(BA)P14K; 5, NHase-(BAP14K). (c) SDS-PAGE of the purified enzymes. 1, marker; 2, wild-type NHase; 3, NHase-(BA); 4, NHase-(BA)P14K; 5, NHase-(BAP14K).