Table 2. Comparison of the catalytic activities of the site-directed mutagenesis variants of the LjFNSII-1.1 and LmFNSII-1.1 enzymes using eriodictyol as the substrate.
| Recombinants | Relative Activity (%) |
|---|---|
| LmFNSII-1.1 | 21.17 (1.08) |
| LmFNSII-1.1-V204M | 222.68 (5.62) |
| LmFNSII-1.1-V379L | 130.61 (13.01) |
| LjFNSII-1.1-M206V | 13.57 (0.88) |
| LjFNSII-1.1-L381V | 30.76 (1.13) |
| LmFNSII-1.1-A120S | 0.35 (0.01) |
| LjFNSII-1.1-S122A | 239.60 (39.62) |
| LjFNSII-1.1 | 100.00 (1.27) |
Relative activity is expressed as a percentage of the activity measured for LjFNSII-1.1. The table shows mean ± SE of three independent experiments.