Figure 5.

The influence of once daily, repeated intrathecal administration of vehicle (“V”, sterile water), LPS-RS [20 μg/5 μL], or LPS-RS Ultrapure [“LPS-RSU”, 20 μg/5 μL] on pain behavior, as measured by von Frey's test (mechanical allodynia; (a), (b)) and the cold plate test (thermal hyperalgesia; (c), (d)), 1 and 3 hours after drug administration on 2nd and 7th days after chronic constriction injury (CCI) to the sciatic nerve. The data are presented as the mean ± SEM (10–25 rats per group). Intergroup differences were analyzed using one-way ANOVA followed by Bonferroni's multiple comparisons test. ⁺ p < 0.05, ⁺⁺ p < 0.01, and ⁺⁺⁺ p < 0.001 indicate significant differences compared with the vehicle-treated, CCI-exposed rat group (V-CCI).