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. 2015 Dec 29;2016:2916017. doi: 10.1155/2016/2916017

Figure 4.

Figure 4

Effects of calyculin A and okadaic acid on the decrease in protein levels of Akt-p (S473) caused by NTG and 8-Br-cGMP. Akt-p (S473) was reduced when treated with NTG (10−6 M; (a)) for 15 min and 8-Br-cGMP (cGMP analog, 10−4 M; (b)) for 60 min. The reduction of Akt-p (S473) was prevented by calyculin A (CA, 10−7 M) but not by okadaic acid (OA, 10−7 M). The upper panels are western blots. The lower panels are the summaries of densitometric scanning of proteins expressed as ratio of Akt-p (S473) to total Akt. Data shown as means ± SEM; n = 6 for each group. ∗significantly different from the control group (P < 0.05).