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Cut-off values for ΔCt calculated by ROC curve analysis
| Reactions | Cut-off values |
|---|---|
| ε2 reaction | 9.2 |
| ε3 reaction | 10.4 |
| ε4 reaction | 11.1 |
The cut-off ΔCt values for the three reactions were calculated from ROC curve analysis, which represent the threshold cycle above which a sample is considered to be negative for the corresponding genotype analysis
